Extended Data Fig. 2: Cryo-EM density-map and comparison of “lid” structures.
From: Cryo-EM structure of the human MT1–Gi signaling complex
a, Cryo-EM density-map of the MT1-ramelteon-Gαi-Gβ1-Gγ2-scFv16 complex (light blue: MT1, yellow: Gαi, green: Gβ1, purple: Gγ2, red: scFv16). The Fab fragment scFv16 binds and stabilizes the interface of Gαi and Gβ1. b, Top view of MT1-Gi and reported lipid GPCRs, viewed from the extracellular side. Receptors (light blue: MT1-Gi, red: TP, blue: EP3, green: EP4) are shown as cylinder models, and ligands are shown as CPK models. MT1-Gi shows the “lid” structure, which is similar to those in the reported crystal structures of lipid GPCRs.
