Fig. 1: Single-particle cryo-EM analysis and model building of the FSE. | Nature Structural & Molecular Biology

Fig. 1: Single-particle cryo-EM analysis and model building of the FSE.

From: Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome

Fig. 1

a, Reference-free 2D class averages. b, Gold standard Fourier shell correlation plots calculated in Relion. c, Reconstructed cryo-EM map in three different views. d, Secondary structure of the FSE as determined by 1D SHAPE chemical mapping. e, The top 10 tertiary structures of the FSE as determined by auto-DRRAFTER using the secondary structure from d, where equivalent structural elements are indicated. These 10 top scoring models had a mean pairwise r.m.s.d. of 5.68 Å, resulting in an estimated accuracy of 5.9 Å based on the previously determined linear relationship23. f, The first strand of stem 1 is captured within a ring formed by the second strand of stem 1 with stems 2 and 3 and J3/2, producing a topologically constrained tertiary fold.

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