Extended Data Fig. 9: PRE-validation of docking model.
From: The structural basis of PTEN regulation by multi-site phosphorylation
Overlay of 15N-1H-HSQC spectra of VSP in presence of 0.9 molar equivalent 4p-T20(TEMPO) in the oxidized (red) and reduced (blue) forms for select peaks of the C2 domain and corresponding intensity ratios plotted against the primary sequence. Green line indicates a ratio of 1, expected for residues away from the spin label. Red line indicates 1*StDev. Segments of 2 or more residues with intensities below the red line are expected in a 20–25 Å radius from the spin label. Notable regions of VSP for their strong interaction or biological relevance are shown in grey areas.
