Extended Data Fig. 6: Mitochondrial transcription is affected by TFAM expression level.
From: Phase separation drives the self-assembly of mitochondrial nucleoids for transcriptional modulation
a, The in vitro transcription of D-loop DNA under the droplet formation condition. The transcription level detected by QPCR under different concentration of TFAM. The bottom panel showed the droplets formation in vitro transcription. Scale bar, 1 µm. b, The transcription level of mitochondria after TFAM knockdown with shRNA (Data are present with mean ± s.d., n = 3 independent experiments). The shA, shB and shC are 3 shRNA targeting TFAM. c, TFAM protein level after TFAM knockdown. d, The mtDNA level after TFAM knockdown by shRNA in Hela cells. Data are present with mean ± s.d., n = 3 technical repeats. e,f, The transcription level (e) and mtDNA copy (f) of mitochondrial after TFAM knockdown by siRNA. g, The distribution of POLRMT after TFAM knockdown by siRNA. POLRMT was labeled by fusion with DsRed, and mtDNA was labeled by SYBR GOLD, while mitochondria were labelled by MitoTracker Deep Red. Scale bar, 10 µm. Data are present with mean ± s.d., and n = 3 independent experiments. Two-tailed unpaired Student’s t-test was used. The p values were noted on the panel. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
