Extended Data Fig. 8: TEFM and MTERF1 alone cannot undergo phase separation.
From: Phase separation drives the self-assembly of mitochondrial nucleoids for transcriptional modulation
a, SDS-PAGE analysis of purified human recombinant TEFM. b, Representative images of TEFM conjugated with Alexa Fluor 568 with or without ND1 DNA under 100 mM NaCl. Scale bar, 5 µm. c, SDS-PAGE analysis of purified human recombinant MTERF1. d, Representative images of MTERF1 conjugated with Alexa Fluor 568 with or without 820 bp Leu DNA containing MTERF1 binding site (tRNA-Leu) under 100 mM NaCl. Scale bar, 10 µm. e,f, The percentage of layered POLRMT after adding TEFM (e) illustrated in (Fig. 4b) or MTERF1 (f) illustrated in (Fig. 4d). g, The super-resolution images of TEFM-DsRed in MEF cells by Hessian-SIM. The mtDNA is labeled with PicoGreen. TFAM is tracked by fusion with EGFP. Scale bar, 0.5 µm. Data are present with mean ± s.d., and n = 3 independent experiments. More than 400 droplets were counted in each group in each experiment. Two-tailed unpaired Student’s t-test was used. The p values were noted on the panel. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
