Extended Data Fig. 9: Mitochondrial transcription after the overexpression of transcription factors or the disturbance of phase separation.
From: Phase separation drives the self-assembly of mitochondrial nucleoids for transcriptional modulation
a, The RNA transcripts in mitochondria was labeled with SYTO RNASelect after the overexpression of TEFM-DsRed or MTERF1-DsRed. Mito, MitoTracker Deep Red. Scale bar, 10 μm. b, The transcription level of mitochondria after the overexpression of TEFM (up panel) or MTERF1 (bottom panel) in Hela cells. Transcription level was detected with QPCR 48 hours after the transfection of 0-0.5 ng plasmids. Transfection with pCAG-FH plasmid was set as control. c,d, The transcription level of mitochondria (c) and TFAM (d) after the overexpression of TFAM truncates in Hela cell meanwhile endogenous TFAM knockdown with siRNA (siTFAM-2). Transcription level was detected with QPCR 48 hours after transfection. The statistical analysis was done by comparation to wild type Hela cells. e, The morphology of TFAM-GFP before or after 10% 1,6-hex treatment for 10 min. Scale bar, 10 µm. f, The transcription level of mitochondria after 1,6-hex treatment. Data are presented as mean ± s.d., n = 3 independent experiments. Two-tailed unpaired Student’s t-test was used. The p values were noted on the panel. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
