Extended Data Fig. 7: Comparison of the core-TRRAP/Tra1 interface in human and yeast.

a. Top view on the hSAGA core (white cartoon) and TRRAP module (gray surface). Parts of the core not in direct proximity to the interface have been removed in the lower panel. Interfacing residues on the TRRAP surface are colored based on their closest core subunit. Interfacing residues of core subunits are shown in stick representation and colored by subunit. b. Magnified region from the box in a. The interface corresponding to the footprint of the core on the TRRAP surface is indicated with a black dashed outline (core interface). The interface created by extensions of core subunits that wrap around the TRRAP is indicated with a blue dashed outline (extended core interface). In hSAGA the latch and SUPT20H CTD (residues 274-428) contribute to the extended core interface. c. Side view of the core and extended interface with the complete core shown as white cartoon. In contrast to ySAGA (f, i), hSAGA has no cleft between the core and TRRAP modules. d,f. Same view as in a-c for S. cerevisiae ySAGA (PDB: 6T9I)⁴. g,h. Same view as in a-c for K. phaffii ySAGA (PDB: 6TB4)⁵. The colored surface regions on TRRAP/Tra1 correspond to the colors of the contacting core subunits and thus show the different participation of core subunits to the interface. f, i. In both ySAGA structures the core and Tra1 modules are separated by a cleft.