Extended Data Fig. 2: Pol I interactions with nucleic acids.
a, Residues contacting the nucleic acid scaffold in human Pol I. The nucleic acid scaffold is presented in cartoon representation with colored circles marking nucleotides visible in the cryo-EM density and open circles marking nucleotides lacking cryo-EM density that have not been included into the final model. Human Pol I residues making contacts with nucleotides within 4 Å radius are depicted. Residues are colored according to the subunits they are a part of and their level of conservation between human and yeast Pol I is marked. b, Comparison of the transcriptional activity between human and yeast Pol I using an RNA primer that dimerizes and a single-stranded RNA primer. Used nucleic acid scaffolds in the cartoon representation are shown on the right. The double-stranded RNA primer (red) has been used throughout the study and the single stranded primer (green) has been designed to have similar base composition. Both primers were radioactively labeled on the 5′ end and annealed to the DNA scaffold as described in Methods. Presence or absence of Pol I in the assay as well as addition of nucleotides is indicated on top.
