Extended Data Fig. 3: Phylogenomic analysis of the stalk subunits and extensions from A135 and ABC23 contacting the stalk. | Nature Structural & Molecular Biology

Extended Data Fig. 3: Phylogenomic analysis of the stalk subunits and extensions from A135 and ABC23 contacting the stalk.

From: Cryo-EM structures of human RNA polymerase I

Extended Data Fig. 3

a, Analysis of A14 and A43 Pol I stalk subunits, smaller stalk subunit RPB4 from Pol II and extensions from subunits A135 (1132–1168) and ABC23 (50–68) as indicated in Fig. 2. Filled square indicates presence of a homologous protein in the corresponding species. Color code as in Fig. 3. Genes are annotated per S. cerevisiae nomenclature. For UniProt identifiers of the identified homologues see Supplementary Table 2. b, Sequence alignment of subunit A135 across species where the S. cerevisiae 1132–1168 insert was identified. Human sequence of RPA2 subunit is included in top row for comparison. Sequences are sorted by alignment score. c, Sequence consensus score plotted across the length of the consensus sequence for the ABC23 subunit. Percentage of sequences with the consensus residue is shown. d, Occupancy plot for the ABC23 subunit showing the number of ungapped residues in each position of the consensus alignment. Total of 48 sequences were aligned. b–d, The red box marks the location of the inserts of interest. Numbering in reference to S. cerevisiae sequence.

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