Extended Data Fig. 4: The distal polyA sites are stronger than the proximal polyA sites.
From: Alternative polyadenylation by sequential activation of distal and proximal PolyA sites
a, Reporter constructed to test the strengths of polyA sites. Fluc and Rluc are Firefly luciferase and Renilla luciferase, respectively. The polyA site (PAS) to be tested is the region ±200 nt flanking the cleavage site. IRES, internal ribosomal entry site. b, Relative luciferase activity of different polyA sites represented by mean ± SD (n = 5). The p-values are based on a two-tailed unpaired t test: ***p < 0.001. c, Constructs with the paired proximal and distal PASs in two different orders. The primers used for RT-PCR were indicated. d, RT-PCR results of two different constructs (C1 and C2) are shown for PASs from 4 genes. Data were from n = 1 independent experiment.
