Extended Data Fig. 1: Nascent RNAs are tightly associated with the nuclear matrix.
From: Alternative polyadenylation by sequential activation of distal and proximal PolyA sites
a, Relative RNA amount extracted from different cellular fractions. Data are presented as mean values ±SD (n = 3). b, Western blotting of the extracts from different fraction with the antibody N20 targeting the largest subunit of RNA polymerase II. Data were from n = 1 independent experiment. c, Histogram of coSI (completed Splicing Index) values of exons. d, Boxplots of coSI values in bins of exons by their distances to the annotated polyA site. e, UCSC genome browser tracks showing the rRNA depleted RNA-seq signals in the nuclear matrix across the genes ADK and GPHN, respectively. The lower and upper hinges of the boxplots correspond to the first and third quartiles (the 25th and 75th percentiles). The upper whisker extends from the hinge to the largest value no further than 1.5 * IQR from the hinge (where IQR is the interquartile range, or distance between the first and third quartiles). The lower whisker extends from the hinge to the smallest value at most 1.5 * IQR of the hinge. The centre line represents the median value.
