Extended Data Fig. 8: AMDE-1 treatment and STING activation stimulate LC3 recruitment to the Golgi. | Nature Structural & Molecular Biology

Extended Data Fig. 8: AMDE-1 treatment and STING activation stimulate LC3 recruitment to the Golgi.

From: ARF GTPases activate Salmonella effector SopF to ADP-ribosylate host V-ATPase and inhibit endomembrane damage-induced autophagy

Extended Data Fig. 8

a, HeLa GFP-LC3 cells stably expressing STING alone or together with GalT-mRuby3 were treated with AMDE-1 or transfected with poly(dA:dT); cells without GalT-mRuby3 expression were stained with anti-TGN46 antibody (red). Fluorescence imaging of the cells are shown (scale bar, 5 µm). b, Stable expression of SopF does not disrupt the Golgi structure. The Golgi in HeLa cells or HeLa cells expressing mCherry-SopF was stained with anti-TGN46 or anti-GM130 antibody (green). Representative images are shown (scale bar, 5 µm). Data are representative of two independent experiments.

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