Extended Data Fig. 1: SopF interactions with ARF proteins.
a, Yeast two-hybrid analysis of SopF–ARF interaction. The assay was performed similarly as that in Fig. 1d except that different ARFs and their indicated mutants were used. b, Myristoylation of ARF1 is not required for binding to SopF. 293T cells co-transfected with Flag-SopF (or Flag-RFP) and the G2A mutant of an indicated ARF. The assay was performed similarly as that in Fig. 1b. c, Co-expression of SopF and the GTP-bound mutant of different ARFs results in formation of stable SopF–ARF complexes, assessed by gel filtration chromatography and Coomassie-stained SDS-PAGE gel. All data are representative of three independent experiments.
