Extended Data Fig. 4: ARLE-14 is required for fertility and nuclear enrichment of MET-2 in met-2-CD animals. | Nature Structural & Molecular Biology

Extended Data Fig. 4: ARLE-14 is required for fertility and nuclear enrichment of MET-2 in met-2-CD animals.

From: SETDB1-like MET-2 promotes transcriptional silencing and development independently of its H3K9me-associated catalytic activity

Extended Data Fig. 4

(a) Brood sizes of the indicated strains at 25 °C. N = 3, n = 60. ns = not significant, p(WT,met-2Δ)< 2.0*10-16, p(WT,met-2-CD) = 4.2*10-6, p(met-2Δ,met-2-CD) < 2.0*10-16, p(met-2Δ, arle-14Δ met-2-CD) = 0.77,by one-way ANOVA, followed by Tukey post-hoc test. (b-c) Live imaging of embryos expressing either WT MET-2::mCherry or MET-2-CD::mCherry + /- arle-14 RNAi grown at 25 °C and (c) quantification of MET-2 nuclear signal. p(WT(vector), WT(arle-14))=not significant, p(WT(vector), met-2-CD(vector))=1.1*10-9, p(WT(vector),met-2-CD(arle-14)) < 2.0*10-16, p(met-2-CD(vector),met-2-CD(arle-14))<2.0*10-16 Low nuclear mCherry signal precluded foci quantification. Scale bar = 5 µm. N = 3 N = 3, n = 45 embryos. nuclei: met-2::mCherry: vector = 884, arle-14 RNAi = 1823, met-2-CD::mCherry: vector = 1076, arle-14 RNAi = 878. ns = not significant, ****p-adj < 10-4 compared to WT vector, ####p-adj < 10-4 compared to MET-2-CD vector by two-sided Wilcoxon signed-rank test.

Source data

Back to article page