Extended Data Fig. 5: Loss of met-2 or heat stress activates transcription in an HSF-1 independent manner.
(a) Quantification of foci loss shown in Fig. 5a, from ref. 14. ****p-adj=1.5*10-9 by two-sided Wilcoxon signed-rank test. (b) Genes de-repressed in both met-2Δ mutant and WT(37 °C) early embryos (Fig. 5d, black circles) are normally expressed in differentiated tissues92. Heatmap shows the expression (RPKM) in WT L3 larvae of each derepressed gene (columns) in individual tissues (rows). (c) Correlation of gene expression in WT and met-2Δ embryos both treated with HS (37 °C, 1 hr) relative to WT(20 °C). Pearson correlation coefficient (r) is displayed. (d) Expression changes of all genes designated Heat Shock Protein (hsp) in WT(37 °C) and met-2Δ relative to WT(20 °C). Pink box = log2 FC > 2 in both WT-HS and met-2Δ−20 °C. (e) Representative images of HSF-1::GFP protein in live WT or met-2Δ embryos + /- HS. HS: 37 °C, 30 min. Scale bar = 5 µm. (f) Fraction of embryos exhibiting HSF-1::GFP foci after 30 min at the indicated temperature. N = 2, n embryos = 20-60 per temperature. (g) Correlation of expression between embryos treated with HS (37 °C, 1 h) and embryos from parents grown at 25 °C6, both compared to WT(20 °C). N = 3.
