Extended Data Fig. 6: Heat stress leads to permissive transcriptional state. | Nature Structural & Molecular Biology

Extended Data Fig. 6: Heat stress leads to permissive transcriptional state.

From: SETDB1-like MET-2 promotes transcriptional silencing and development independently of its H3K9me-associated catalytic activity

Extended Data Fig. 6

(a) Comparison of H3K9me2 genome wide enrichment (500 bp tiles) over input (log2) between HS (37 °C, 60 min) and WT(20 °C) embryos via ChIP-seq (N = 2). Boxplots show median, boxes 50% and whiskers 90% of each group. Correlation between replicates: HS = 0.97, WT(20 °C) = 0.98. (b) Percentage of defined genomic elements that overlap with peaks differentially enriched for H3K27ac in HS treatment (37 °C, 60 min), met-2Δ, and met-2-CD embryos relative to WT(20 °C). (c) Representative Western blot and quantification of MET-2::mcherry in embryos + /- HS: 37 °C, 60 min. N = 3. *p = 0.050 by two-sided t-test. (d-e) Log2 FC of met-2Δ-specific genes, met-2Δ»met-2-CD, and common genes in HS (37 °C, 60 min), met-2Δ and met-2-CD and (d) mean fold change observed in HS in each gene set. N = 3. Boxplots show median, boxes 50% and whiskers 90% of each group. (f-g) Correlations between RNAseq replicas for (e) WT and met-2Δ + /- HS (37 °C, 60 min), and (f) WT, met-2Δ, met-2-CD, arle-14Δ, and arle-14Δ met-2-CD.

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