Extended Data Fig. 1
a, Western blot analysis showing expression levels of indicated Gal4-DBD fusion proteins following transient transfection in OSCs (Ponceau S-levels indicate protein loading; related to Fig. 1b). b, H3K9me3 levels (normalized to heterochromatic light locus) at the reporter locus (amplicon indicated in Fig. 1a) determined by ChIP-qPCR from OSCs expressing indicated Gal4-DBD fusion proteins (data are presented as mean of n = 2 biological replicates). c, Western blot analysis showing expression levels of Gal4-DBD fusion proteins with indicated Panx IDR fragments (amino acid boundaries indicated). Ponceau S levels indicate protein loading; related to Fig. 1d. d, Western blot analysis showing expression levels of Gal4-DBD fusions with indicated WT and LxxLL mutant Panx fragments. Ponceau S levels indicate protein loading; related to Fig. 1e. e, To the left: Boxplots showing GFP reporter levels in OSCs following transfection with plasmids encoding Gal4-DBD fusions of Panx peptides (wildtype versus alanine mutant; numbers indicate median fold repression normalized to control for two biological replicates, boxplots indicate median, first and third quartiles (box), whiskers show 1.5× interquartile range; outliers were omitted; n = 10,000 cells). To the right: Western blot analysis showing expression levels of Gal4-DBD fusion proteins with indicated Panx peptides. Ponceau S levels indicate protein loading.
