Extended Data Fig. 2

a, Confocal image of egg chambers with indicated germline-specific knockdown (GLKD) stained for Sov (greyscale; scale bar: 20 μm). b, H3K9me3 Cut&Run signal from OSCs with indicated knockdowns at indicated transposons (The Doc retroelement serves as a control transposon as it is not targeted by the piRNA pathway in OSCs). c, Sov-GFP ChIP–Seq signal at indicated transposon families from OSCs expressing Sov-GFP or not (control) (The Doc retroelement serves as a control transposon family as it is not targeted by the piRNA pathway in OSCs). d, Western blot analysis showing expression of Gal4-DBD-FLAG-Sov following plasmid transfection in the OSC reporter cell line (related to Fig. 2f,g).