Extended Data Fig. 7: GPIT activity detected in flow cytometry experiment.
From: Structure of human glycosylphosphatidylinositol transamidase
a, Cartoon scheme of GPIT activity analysis using GSTCy5-nanobodyGFP. b, The gating strategy for FACS. HEK293T cells was first gated on FSC and SSC signals (R1). GFP and Cy5 positive cells were defined as cells exceeding fluorescence of wild type cells (R2). c, The HEK293 cells (WT or PIGK deficient) expressing GFP-GPI and PIGK (WT or mutants) are analyzed according to GFP and Cy5 fluorescence using flow cytometry. Cells was first gated on FSC and SSC signals. GFP and Cy5 positive cells were defined as cells exceeding fluorescence of wild type cells. The flow cytometry experiment was repeated at least five times.
