Extended Data Fig. 1: Purification of the human GPIT complex.
From: Structure of human glycosylphosphatidylinositol transamidase
a, Size−exclusive chromatography (Superose 6 increase) of purified sample of human GPIT complex. Peak fractions within the dashed lines were collected for cryo-EM sample preparation. b, Coomassie−blue-stained SDS-PAGE gel of SEC peak fractions. GPIT components are labeled. The bands of PIGS and PIGK are smeared because of varied extent of glycosylation. The experiments were repeated independently more than 3 times with similar results.
