Extended Data Fig. 3: Cryo-EM data processing of the human GPIT complex.

a, Flow chart of cryo-EM data processing of the human GPIT complex. A total of 2,186 movie stacks were collected (Bar = 40 nm). A representative motion-corrected micrograph of this dataset is shown here. Particles were picked using Gautomatch, Topaz, and Template Picker in cryoSPARC, and were then submitted to several rounds of 3D classification, Bayesian polish, and CTF and 3D-auto refinement. The final map was reported at 3.1 Å according to GSFSC criterion. b, Sharpened map of human GPIT complex, colored according to the estimation of local resolution. c, Angular distribution of particles contributed to the final reconstruction. The length of cylinders indicates the number of particles in the designated orientation. d, Half-map and model-map FSC curves of human GPIT complex. Half-map FSC curves were calculated before (red) and after (blue) PostProcesss, overlaid with a model-map FSC which is calculated between the final map and structural model. e, Representative EM maps for the human GPIT complex and the GPI molecule.