Fig. 4: Cryo-EM structure of PI3KC2αΔN.
From: Structural basis of phosphatidylinositol 3-kinase C2α function
a, The selected 2D classes of PI3KC2αΔN from Extended Data Fig. 5b. The selected 2D classes without or with diffused density of PX domain are labeled with white or red stars. The density of PX domain is shown with a magenta arrow. The donut-shaped C-C2 domain is indicated with a purple arrow. Scale bar, 20 nm. b,c, 3D cryo-EM map of PI3KC2αΔN overlaid onto the crystal structures of PI3KC2αΔN+ΔC−C2 and PI3KC2αcore and the C-terminal C2 domain of human PI3KC2α (PDB 6BTY). The flexible PX domain is shown as a transparent ribbon, where the EM density was subtracted after 3D reconstruction. The overall structure of PI3KC2αΔN reveals a closed conformation of the enzyme.The views in b and c are related by 90° rotation.
