Extended Data Fig. 1: Purification and lipid kinase activity of PI3KC2αΔN, PI3KC2αΔN+ΔC−C2, and PI3KC2αcore. | Nature Structural & Molecular Biology

Extended Data Fig. 1: Purification and lipid kinase activity of PI3KC2αΔN, PI3KC2αΔN+ΔC−C2, and PI3KC2αcore.

From: Structural basis of phosphatidylinositol 3-kinase C2α function

Extended Data Fig. 1

(a) Size exclusion chromatography of PI3KC2αΔN, PI3KC2αΔN+ΔC−C2 and PI3KC2αcore. Peak fractions were collected for structural analysis. (b) Coomassie Blue-stained SDS–PAGE of purified PI3KC2αΔN, PI3KC2αΔN+ΔC−C2 and PI3KC2αcore from (a). (c) In vitro kinase activity of PI3KC2αWT and PI3KC2αΔHBD using PI(4)P as a substrate. HBD deletion showed no influence on kinase activity. Data represent mean ± SD from triplicate (n = 3) experiments. One sample, 2 tailed t-test with hypothetical mean of 100, NS = non-significant.

Source data

Back to article page