Extended Data Fig. 2: Secondary structure of PI3KC2α and primary sequence alignment with PI3Ks.
From: Structural basis of phosphatidylinositol 3-kinase C2α function
The sequence corresponding to crystallization construct I (that is musPI3KC2αΔN+ΔC−C2) was used as template to align human PI3KC2α (hs PI3KC2α, UniProt accession: O00443.2), other human class II PI3K isoforms [PI3KC2β (UniProt accession: O00750.2), PI3KC2γ (UniProt accession: O75747.3)], human class I PI3K (PI3Kγ, UniProt accession: P48736.3), and human class III PI3K (VPS34, UniProt accession: Q8NEB9.1). The dashed lines indicate disordered linker regions. All identified secondary structural elements are color-coded as in Fig. 1a. The light grey arrow indicates the replacement of a short, disordered sequence by GSGS to improve crystallization of PI3KC2αΔN+ΔC−C2. To generate the ΔHBD construct, the HBD was replaced by the sequence SGAGSGA as indicated by the orange arrow. kα12 (N) is indicated by a black arrow.
