Table 2 Cryo-EM data collection statistics
From: Structural basis of phosphatidylinositol 3-kinase C2α function
PI3KC2α (untilted) (EMDB-12191) | PI3KC2α (−30° tilted) (EMDB-12191) | |
|---|---|---|
Data collection and processing | ||
Magnification | 105,000 | 105,000 |
Voltage (kV) | 300 | 300 |
Electron exposure (e–/Å2) | 60 | 60 |
Defocus range (μm) | −1.5 to −2.8 | −1.5 to −2.8 |
Pixel size (Å) | 0.837 | 0.837 |
Symmetry imposed | C1 | C1 |
Initial particle images (no.) | 2.3 million | 452,000 |
Final particle images (no.) | 601,000 (untilted + tilted) | |
Map resolution (Å) | 4.4 | |
FSC threshold | 0.143 | |
Map resolution range (Å) | 5.2–4.4 | |
