Extended Data Fig. 7: Ada3 acetylation-dependent SAGA dimerization is required for gene transcription under stress conditions.
a, Ada3 acetylation is required for PHO5 transcription when grown in phosphate starvation media. b, Bromodomains of Gcn5 and Spt7 are required for PHO5 transcription when grown in phosphate starvation media. c, GSEA analysis of cell cycle related genes in Ada3-3KR, Gcn5 Bro-mut and Spt7 Bro-mut. GSEA uses the expression information of all genes through clusterprofiler (R package). P value was calculated by permutation test based on the gene set. d, Flow cytometry analysis of cell cycle profiling of WT, Ada3-3KR and Gcn5/Spt7 Bro-mut when grown in glucose-containing medium. e, Volcano plots for differentially expressed genes by sucrose treatment from RNA-seq experiments. The edgeR is used to calculate P value. f, Ada3 acetylation is required for SUC2 transcription when grown in sucrose-containing media. For Extended Data Fig. 7a, b, f, data represent means ± SE; n=3 biological independent experiments; two-sided t-tests were used for statistical analysis.
