Extended Data Fig. 10: The interaction between SAGA and transcription activators and TBP is unaffected by Ada3 acetylation in vitro. | Nature Structural & Molecular Biology

Extended Data Fig. 10: The interaction between SAGA and transcription activators and TBP is unaffected by Ada3 acetylation in vitro.

From: Acetylation-dependent SAGA complex dimerization promotes nucleosome acetylation and gene transcription

Extended Data Fig. 10

a GST pull-down assays with purified GST-Gal4 and purified WT SAGA and SAGA Ada3-3KR. GST only acts as a negative control. b GST pull-down assays with purified GST-Gcn4 and purified WT SAGA and SAGA Ada3-3KR. GST only acts as a negative control. c In vitro Co-IP with purified TBP and purified SAGA WT Ada3-FLAG and SAGA Ada3-3KR-FLAG. SAGA WT Ada3-FLAG and SAGA Ada3-3KR-FLAG were immunoprecipitated with anti-FLAG beads and TBP was detected with anti-TBP antibody. For Extended Data Fig. 10a-c, shown is the typical example of two biological independent experiments.

Source data

Back to article page