Extended Data Fig. 5: The data-processing workflow of bison NHA2 ΔN in nanodiscs.
From: Structure, mechanism and lipid-mediated remodeling of the mammalian Na+/H+ exchanger NHA2
a. The dataset contained 17,783 movies that were corrected by Patch motion correction and Patch CTF estimation in cryoSPARC. After template based auto-picking, 3,055,998 particles were selected. Several rounds of 2D classification were performed, yielding 838,438 particles, which were subjected to 3D classification. One of the three 3D main classes was selected, and it contained 563,493 particles. After non-uniform refinement in cryoSPARC, a resolution of 3.61 Å was achieved that was improved with local refinement and masking to 3.22 Å and after SIDESPLITTER in Relion a resolution of 3.5 Å was achieved at the gold standard FSC (0.143), with a local resolution range of 3.4 to 4.6 Å. b. Angular distributions of the final reconstruction for NHA2ΔN in nanodiscs c. The FSC curve for the NHA2ΔN in nanodiscs (PDB:7P1K).
