Extended Data Fig. 7: Tau PFFs fail to induce tau pathology in Tau-/- mice.
From: Tau modification by the norepinephrine metabolite DOPEGAL stimulates its pathology and propagation
Pre-formed fibrils (PFFs) of Tau or Tau K353R were injected into the LC of 3-month-old Tau -/- mice. The mice were assessed for DOPEGAL modification and phosphorylation of Tau 3 months later. A, B. Representative images of immunofluorescent staining for Tau K353-DOPEGAL (red); DBH (green); DAPI (blue) (A) and Tau K353-DOPEGAL (green); AT8 (red); DAPI (blue) (B). Scale bars are 100 μm (left) and 20 μm (right). C. Quantification of DBH + cells in the LC sections of PBS, Tau, or Tau K353R PFFs-injected Tau-/- mice. Data are shown as mean ± SEM. n = 8 per group. One-way ANOVA. D. Quantification of Tau K353-DOPEGAL + and AT8 + cells in the LC sections of PFFs-injected Tau -/- mice. Data are shown as mean ± SEM. n = 6 per group. Two-way ANOVA with Sidak’s multiple comparison.
