Extended Data Fig. 1: Biophysical characterization of recombinant Tau and Tau K353R mutant fibrils.
From: Tau modification by the norepinephrine metabolite DOPEGAL stimulates its pathology and propagation
A. X-ray diffraction assay demonstrating characters of fibrils generated from Tau, Tau K353R, Tau N368, and Tau N368 K353R protein. B. Quantification table showed that DOPEGAL increased the counts of β-sheet at around 4.8 Å for Tau and Tau N368, but not Tau K353R and Tau N368 K353R. C. LC-MS/MS spectrum confirming DOPEGAL-modified Tau residue at K353 by the application of GluC digestion for the protein samples. D. Recombinant wild-type and K353R Tau were induced to aggregate into PFFs in the presence or absence of DOPEGAL. The PFFs were digested with Protease K (2 μg/ml). All data are representatives of three independent experiments with similar results. E. Recombinant Tau and Tau N368 were incubated with DOPEGAL (500 μM) for 0, 5, 30, and 60 minutes. Western blot and densitometric quantification of TauK353-DOPEGAL band showed that Tau N368 is more prone to be modified by DOPEGAL than full-length Tau. All data are shown as mean ± SEM. n = 3 per group. Paired Student t-test.
