Extended Data Fig. 6: AP2core-heparin-SGIPAPA symmetry expansion and SGIPAPA model building. | Nature Structural & Molecular Biology

Extended Data Fig. 6: AP2core-heparin-SGIPAPA symmetry expansion and SGIPAPA model building.

From: Structural basis of an endocytic checkpoint that primes the AP2 clathrin adaptor for cargo internalization

Extended Data Fig. 6

(a) Density that is not accounted for by AP2 subunits in the AP2core-heparin-SGIPAPA structure is shown in pink. Density on the surface of β2 we assign to SGIPAPA as it is not observed in AP2-heparin structures and all residues at the α and β2 termini are accounted for. (b) Masks used for partial signal subtraction and manual symmetry expansion. (c) AP2-heparin-SGIPAPA ‘monomer’ refinement using signal subtracted particles. A map filtered by local resolution is shown colored by local resolution (left). The sharpened map is shown (grey) along with the FSC plot. (d) The poly-ALA model of SGIPAPA is shown in the density between β2 helices 25 and 27.

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