Extended Data Fig. 10: Examples of three-color fluorescence imaging.
(a) Five lipid-coated beads are shown in three separate imaging channels and merged. Beads contain 2% YxxΦ-cargo and were incubated with 1 µM FCHoBAR and 1 µM AP2core. (b) Five example beads are shown as in (A), but were incubated with 1 µM FCHoBAR+APA. (c) and (d) show the same experiment in (A) and (B), but using SGPMP+APA. (e) Quantification of beads containing YxxΦ-cargo puncta with or without SGIPMP+APA Data are mean +/- sd of duplicate measurements. ****P < 0.0001 for unpaired t-test (f) Quantification of the AP2 to lipid ratio in cargo puncta with or without SGIPMP+APA. Data are mean +/- sd of triplicate measurements. ****P < 0.0001 for unpaired t-test. Data for graphs in e,f are available as source data. Scale bar: 5 µm.
