Extended Data Fig. 6: Details of DNA cyclization experiments.
a, Fluorescence image and analysis of native PAGE gel resolving ligation products. Gel represents one replicate. Three replicates are plotted on the graphs. The polymeric/cyclized band assignments were made by reference to the relative electrophoretic mobilities observed in Kahn & Crothers, 1992. b, Comparison of Cas9:DNA cyclization data to CAP:DNA cyclization data. The depicted model is \(y = A \cdot \sin \left( {\frac{{2\pi }}{{10.45\ bp}}\left( {x + \phi _0} \right)} \right) + b\), with the following constraints: \(A > 0\), \(b > A\). The average of 224° and 212° is reported in Fig. 4c. J, J-factor (defined in Kahn & Crothers, 1992); Φ, phase difference; CBS, CAP-binding site.
