Extended Data Fig. 7: Details of permanganate reactivity measurements.
a, Autoradiographs and analysis of all thymines except T(+25), which was insufficiently resolved from neighboring bands. The depicted autoradiographs are replicate 1. Due to systematic variation across replicates, individual replicates are presented on separate graphs and fitted separately. The depicted model is \(P_{ox} = \frac{{B_{\max }[Cas9:sgRNA]}}{{K_D + [Cas9:sgRNA]}}\), with KD shared across T(+1) and T(+2). CCR, candidate complementarity region; CI, confidence interval. b, Autoradiograph (same gel as ‘intact PAM’ autoradiograph in a) and analysis of experiments containing variants of Cas9:sgRNA (16 μM), with the ‘intact PAM’ DNA substrate. Graph depicts three replicates.
