Extended Data Fig. 5: Synchronization of ICL unhooking by NEIL3-depletion and add back.
From: The HMCES DNA-protein cross-link functions as an intermediate in DNA interstrand cross-link repair
a, NEIL3 and HMCES immunodepletion. The extracts used in the replication reactions shown in Fig. 3b were blotted for HMCES and NEIL3. Asterisks, non-specific bands. b, pICLAP was replicated in the presence of [α-32P]dATP in the indicated extracts (shown in a) supplemented with p97i for 60 min. rNEIL3 was then added to the reactions to allow ICL unhooking. Replication intermediates were analyzed as in Fig. 2a. c, HMCES immunodepletion. The extracts used in Fig. 4 were blotted for HMCES. Asterisk, non-specific band.
