Extended Data Fig. 2: HMCES suppresses DSB formation specifically during NEIL3-dependent ICL repair.
From: The HMCES DNA-protein cross-link functions as an intermediate in DNA interstrand cross-link repair
a, b, d, f, and h, HMCES and NEIL3 immunodepletions. The extracts used in Fig. 2a (a), Extended Data Fig. 2c (b), Extended Data Fig. 2e (d), Extended Data Fig. 2g (f), and Fig. 2c (h) were blotted for HMCES and NEIL3 as indicated. Asterisks, non-specific bands. c, pICLAP was replicated with [α-32P]dATP in mock- or HMCES-depleted extracts. Replication intermediates were analyzed as in Fig. 2a alongside pCtrl replication products that were linearized by digestion with HincII. e, pICLAP was replicated with [α-32P]dATP in mock- or HMCES-depleted extracts supplemented with rHMCES, as indicated. Replication intermediates were analyzed as in Fig. 2a. g, pCtrl, pICLPt, or pICLAP were replicated with [α-32P]dATP in mock- or HMCES-depleted extract and replication intermediates were analyzed as in Fig. 2a.
