Extended Data Fig. 8: Thermal shift assay of phosphorylated (as purified) versus dephosphorylated (PP1 treated) GYS1:GYG1 complexes in the presence of increasing concentrations of Glc6P. | Nature Structural & Molecular Biology

Extended Data Fig. 8: Thermal shift assay of phosphorylated (as purified) versus dephosphorylated (PP1 treated) GYS1:GYG1 complexes in the presence of increasing concentrations of Glc6P.

From: Molecular basis for the regulation of human glycogen synthase by phosphorylation and glucose-6-phosphate

Extended Data Fig. 8

a, Gel shift of GYS1:GYG complexes mock (M) or treated with PP1c (+) for 2 hours at room temperature. 5 µg of each complex was loaded and ran on SDS-PAGE. A decrease in the molecular weight of GYS1 after PP1 treatment is apparent. b, Thermal shift assay of GYS1:GYG1FL against Glc6P. c, Thermal shift assay of GYS1:GYG1p.Y195F against Glc6P. Median melting temperatures and standard deviations are shown (n = 4).

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