Extended Data Fig. 3: Estimation of condensin-binding position on supercoiled DNA measured with AFM.
a) Representative AFM images showing condensin binding near a plectoneme body (left) and near the plectoneme tip (right). Data represent 4 independent experiments. b) Statistics showing enrichment of condensin as observed outside of plectoneme, at plectoneme body, and at apical loops. In order to avoid possible supercoiling induced by condensin, the plasmids were incubated with AMP-PNP.
