Extended Data Fig. 4: Additional examples showing that condensin initially loads near a plectoneme tip and moves downwards during plectoneme loop extrusion. | Nature Structural & Molecular Biology

Extended Data Fig. 4: Additional examples showing that condensin initially loads near a plectoneme tip and moves downwards during plectoneme loop extrusion.

From: Condensin-driven loop extrusion on supercoiled DNA

Extended Data Fig. 4

a) Snapshots showing condensin moving from the plectoneme tip (1st) towards the stem (till 3rd snapshot). Simultaneously the plectoneme becomes compacted and moves together with the condensin during extrusion while it grows back its length at later times (3rd – 5th frame). Data represent 3 independent experiments. b) Snapshot (left) and kymograph (right) showing condensin moving from the plectoneme tip towards the stem. Data represent 3 independent experiments. (c) Change in position for condensins that bound at the tip and moved along the length of DNA plectonemes extracted from the kymographs as in panel b for N = 6 molecules. The red colored trace corresponds to the kymograph in panel b.

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