Extended Data Fig. 7: The SLX4 MLR domain contributes to I-SceI-induced LTGC. | Nature Structural & Molecular Biology

Extended Data Fig. 7: The SLX4 MLR domain contributes to I-SceI-induced LTGC.

From: The structure-specific endonuclease complex SLX4–XPF regulates Tus–Ter-induced homologous recombination

Extended Data Fig. 7

a, b. Tus/Ter-induced LTGC (a) and I-SceI-induced LTGC (b) in Slx4+/– and Slx4∆MLR/– clones. Data shows mean values of five biologically independent replicates, n = 5. P-value: **p < 0.01. Error bars: s.e.m. Analysis by ordinary one-way ANOVA. Error bars: s.e.m. c and d. Impact of exogenous hSLX4 alleles (see Fig. 5c) on Tus/Ter-induced LTGC (c) and on I-SceI-induced LTGC (d) in Slx4+/– and Slx4∆UBZ/– clones. EV: empty vector. 4 C > A: hSLX4 with inactivated UBZ motifs. Data shows mean values of six biologically independent replicates, n = 6. P-value: *p < 0.05; **p < 0.01; ***p < 0.00. Error bars: s.e.m. Analysis by unpaired Student’s t-test.

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