Extended Data Fig. 2: Structural details of the export-competent Bud20-TAP pre-60S particle (rRNAs that are not highlighted are displayed as grey surfaces).
From: Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1
(a) Drg1 docking platform composed of Arx1 and ES27. (b) Localization of the ribosomal protein Rpl12 (L12) and the shuttling factors Nog1, Nsa2, Mrt4, Nmd3, Nog1 as well as 25 S rRNA helices 38 (h38) and 89 (h89). (c) Closed L1 stalk. Localization of ribosomal protein L1. (d) The N-terminus of Nmd3 is not resolved in the structure. The black arrow marks the first resolved residue of Nmd3 (R156). (e) and (f) Entanglement of Bud20, Rlp24, Nog1 and Tif6. (g) Localization of the two N-terminal Nsa2 helices (His20 to Lys74) between h89 and Mrt4. (h) Interaction surface between Nsa2 and 25 S rRNA helix 89. (i) Nsa2 connects Mrt4 and helix 89.
