Extended Data Fig. 3: Non-planar architecture of two representative huPrP23-144 protofilaments assembled through large hydrophobic interfaces.
From: Cryo-EM structure of disease-related prion fibrils provides insights into seeding barriers
Subunit n in protofilament A interacts with two subunits in protofilament B (subunits m and m + 1); subunit m + 1 in protofilament B interacts with two subunits in protofilament A (subunits n and n + 1). Such a non-planar conformation results in rugged surfaces at fibril ends, with N-terminal residues at the top end and C-terminal residues at the bottom end exposed to water. A similar non-planar assembly is observed for subunits in protofilaments C and D.
