Extended Data Fig. 6: Analysis of SHOC2 binding to the surface of MRAS.
From: Structure of the SHOC2–MRAS–PP1C complex provides insights into RAF activation and Noonan syndrome
a The N-terminal LRRs of SHOC2 (pink) are shown interacting with the switch I (dark blue) and switch II (purple) of MRAS (blue). b The C-terminal LRRs of SHOC2 (pink) are shown interacting with the C-terminus of MRAS (blue surface). c Residues of MRAS found mutated in NS highlighted as spheres on the structure of MRAS. d Single-cycle kinetic analysis was performed on immobilized avi-tagged SHOC2 mutants as denoted in the figure with five injections of MRASGMPPNP and PP1CA (blue). The data were fit to a 1:1 kinetic model (black).
