Extended Data Fig. 10: Two-electrode voltage clamp recordings of mouse HCN2.
From: Anionic lipids unlock the gates of select ion channels in the pacemaker family
(a) TEVC recordings of oocytes expressing HCN2 after treatment with PMA (top panel) or Wortmannin (bottom panel). (b) TEVC recordings of oocytes expressing HCN2 D443N after treatment with PMA (top panel) or Wortmannin (bottom panel). Zoom panels in A and B show tail currents that were used for analysis to determine Itail/Itailmax. Recordings at −75 mV are in red. (c) Voltage dependent activation of HCN2 (open symbols, dashed lines) and HCN2 D443N (closed symbols, lines) before (black) and after treatment of the oocytes with Wortmannin (orange, for WT HCN2 n = 7, for HCN2 D443N n = 4). Data points are average ± s.d., lines are fits according to equation 3. Fits for untreated oocytes are from Fig. 4f. Values are summarized in Supplementary Table 1.
