Extended Data Fig. 5: The NTD modeling and sequence alignments among human NaV channels.
From: Structural basis for NaV1.7 inhibition by pore blockers
a. The un-sharpened and sharpened (using DeepEMhancer) EM maps of NaV1.7XEN. b. The EM map of the N-terminus domain (NTD) was shown in surface, the secondary structure α1, α2, β1, β2 and amino acids with good density were displayed and labeled. c. AlphaFold2 model of NaV1.7 NTD. d. Structural comparison of the NaV1.7 NTD and its AlphaFold2 model. e. Gain-of-function, loss-of-function and other pathogenic mutation sites in the NTD of NaV channels are highlighted in green, red and gray, respectively. Secondary structure elements are indicated on top of the sequences. R116 in S0I of NaV1.7 mediates key interactions between the NTD and VSDI is identical among the isoforms.
