Extended Data Fig. 2: Cryo-EM data processing of NaV1.7-antagonist complexes.
From: Structural basis for NaV1.7 inhibition by pore blockers
a. The workflow of cryo-EM data processing. A total of 2,900, 2,601 and 4,430 movie stacks were collected for NaV1.7-β1-β2-XEN907 (orange), TC-N1752 (blue), and NaV1.7-IN2 (pink) respectively. Particles were auto-picked in Relion3, 2D and 3D classifications were conducted to remove bad particles, followed by 3D AutoRefine in Relion3. Subsequent Polish and CTF Refine improved image quality. According to the gold-standard Fourier Shell Correlations (FSC) criterion, the final maps were determined to 3.22, 3.09, and 3.07 Å, respectively. b-d, Sharpened map of the NaV1.7XEN (b), NaV1.7TCN (c) and NaV1.7IN2 (d) complex, colored according to the local resolution values (left). Particle angular distribution for the final 3D reconstruction (middle). FSC of the final map of the complex, calculated between two independently refined half-maps before (blue) and after (red) post-processing, overlaid with an FSC curve calculated between the cryo-EM density map and the structural model shown in black (right).
