Extended Data Fig. 10: dCas9 and dCas12a removal efficiency during transcription in the presence of GreB. | Nature Structural & Molecular Biology

Extended Data Fig. 10: dCas9 and dCas12a removal efficiency during transcription in the presence of GreB.

From: Polarity of the CRISPR roadblock to transcription

Extended Data Fig. 10

The same data taken for Fig. 2c were used for this analysis, so the sample trace statistics are identical to those for Fig. 2c. Out of the removal efficiency, the fraction from traces with removal due to transcription read-through (grey) and the fraction from traces with removal but without transcription read-through (red) are stacked. Black dots are removal efficiencies calculated for each independent replicate. Error bars are SEMs. Source Data File for these bar graphs is provided.

Source data

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