Fig. 1: Reconstituted, functional PIC–nucleosome complex.
From: Structures of transcription preinitiation complex engaged with the +1 nucleosome
a, Schematic of template DNA. The distances between the TATA box, TSS and nucleosome dyad are indicated. The expected lengths of template and transcript are noted at the bottom right. b, Schematic of in vitro transcription assay. For details, see the Methods. c, The +1 nucleosome impairs promoter-dependent transcription in vitro. Assays were performed with template DNA without (DNA) or with +1 nucleosome (Nuc). Representative original scan of the urea–PAGE analysis that yielded results presented on the right. RNA transcripts were analyzed by urea-denaturing PAGE and the full-length product was quantified (dashed rectangle). Experiments were performed at least three times. Bars correspond to the mean of three independent experiments; error bars represent the s.d. FL, full-length transcript; ST, shorter transcripts.
