Extended Data Fig. 1: In vivo Cgt activity assay.
From: Mechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella
a, Growth dynamic of Atu under osmotic stress. The Δcgt mutant can be rescued with WT B. abortus cgt gene, but not with cgtE497Q. Addition of 100 mM NaCl restores growth of all strains. b, Atu Δcgt was rescued with different point mutants of Bab cgt under low osmotic stress conditions (YPL). c, Same as b, with low osmotic stress partially alleviated by the addition of 15 mM NaCl to the medium. d, Cartoon representation of the constructs used in the assay. e, Western blot analysis of cgt-3xFlag gene expression in Atu strains used in the assay. All growth curves represent mean values of 3 independent experiments (n = 3). Error bars represent standard deviation. Data for graphs a-c and uncropped images for e are available as source data. Western blot analysis was performed once (n = 1).
