Extended Data Fig. 2: DHT03 can undergo different loop connectivities to form alternate desired oligomers.
From: De novo design of obligate ABC-type heterotrimeric proteins
Different loop closures were applied to DHT03 to create a total of five heterodimers, one alternate ABC heterotrimer, and one ABCD heterotetramer, as determined by native mass spec. The alternate heterotrimer and ABCD heterotetramer were made by removing the existing loop on the chain C helical hairpin, resulting in single helices for each chain. Black solid lines represent loops facing all on the same side, while dashed lines represent loops on opposite ends of termini.
